Figure 3.

Consecutively OS-Elicited WRKY-Silenced Plants Show Decreased JA and JA-Ile/-Leu Levels.

Mean (±se) of JA ([A] and [B]) and JA-Ile/-Leu ([C] and [D]) concentrations in OS-elicited leaves of separate plants of the wild type (black), two ir-wrky3 lines (gray), and two ir-wrky6 lines (white).

(A) and (C) Leaves harvested 60 min after a single OS elicitation, during which one leaf with six rows of holes was treated with 20 μL of OS (n = 5/genotype; * P < 0.05, as determined by Fisher's PLSD test from ANOVA).

(B) and (D) Leaves harvested after multiple OS elicitations, whereby one leaf was wounded with one row of holes and 10 μL of OS was applied to the wounds. Treatment was repeated six times every 30 min, and leaves were harvested 30 min after the last elicitation (n = 5/genotype; * P < 0.05, as determined by Fisher's PLSD test from ANOVA).