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. 2008 Jul;20(7):1984–2000. doi: 10.1105/tpc.108.058594

Figure 4.

Figure 4.

In Field-Grown Plants, Silencing WRKY3, WRKY6, or Both Reduces Constitutive and OS-Elicited Levels of JA.

(A) Mean (±se) concentrations of JA in leaves of wild-type (solid line), ir-wrky3 (dashed line), ir-wrky6 (dotted line), and ir-wrky3/6 (dashed/dotted line) plants grown in the Great Basin Desert in Utah after a single elicitation with OS. Mean (±se) LOX3 transcript levels of wild-type (solid line), ir-wrky3 (dashed line), ir-wrky6 (dotted line), ir-wrky3/6 (dashed/dotted line) plants (n = 6/harvest and genotype). Mean (± se) of AOS, AOC, and OPR3 transcript levels before (white) and 30 min after (black) OS elicitation (n = 6/harvest and genotype). Different letters reflect significant differences at P < 0.05 among plants that underwent the same treatment. The transcripts were analyzed by real-time PCR.

(B) Mean (±se) concentrations of JA-Ile/-Leu measured as above. Mean (±se) of Na TD and Na JAR4 transcript levels before (white) and 60 min after (black) OS elicitation (n = 6/harvest and genotype).