Figure 6.

Impaired Direct and Indirect Defenses Are Restored by Applying JA, and Plants Silenced in WRKY3 Are Delayed in JA-Ile Conjugation.

(A) Mean (±se) of TPI activity measured after plants were elicited with JA-supplemented OS (10 μg/μL, n = 6) and harvested 3 d later.

(B) Mean (±se) emissions of cis-α-bergamotene from wild-type plants (black), lines silenced in WRKY3 (dark gray), WRKY6 (light gray), or both (open) 24 h after plants were treated with JA-supplemented OS (10 μg/μL).

(C) Larval mass of M. sexta that fed on wild-type plants (solid line), WRKY3 (dashed line), WRKY6 (dotted line), and WRKY3/6-silenced (dashed/dotted line) glasshouse-grown plants sprayed with 1 mM JA solution (diluted in 30% ethanol) (+JA; n = 12) or sprayed with 30% ethanol as control (inset; mock, n = 12)

(D) Mean (±se) of JA-Ile conjugation after adding both exogenous JA (0.625 μmol) and Ile (0.625 μmol) to the OS in the greenhouse (n = 4, * P < 0.05, as determined by Fisher's PLSD test from ANOVA).