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. Author manuscript; available in PMC: 2008 Aug 20.
Published in final edited form as: J Am Chem Soc. 2007 Mar 9;129(13):3812–3813. doi: 10.1021/ja069098t

Table 1.

Kinetic Constants of TCPTP Insertion Mutants

Protein kcat(s−1) KM for pNPP(mM) kcat/KM(s−1mM−1)
WT TCPTP 3.9±0.20 2.3±0.21 1.7
TCPTP-65 2.8±0.13 2.9±0.16 1.0
TCPTP-78 1.5±0.048 1.8±0.058 0.84
TCPTP-79 5.0±0.043 1.2±0.046 4.1
TCPTP-80 1.1±0.050 2.0±0.24 0.56
TCPTP-187 0.21±0.0084 0.91±0.059 0.23
TCPTP-188 0.25±0.012 1.1±0.12 0.23
TCPTP-208 1.3±0.032 3.5±0.14 0.38
TCPTP-209 0.41±0.017 1.8±0.13 0.23
TCPTP-241 2.1±0.15 2.2±0.29 1.0
TCPTP-CT 1.2±0.034 2.2±0.084 0.54