Table 1.
Methylobacterium Strains and Plasmids Used in This Study
| Strain/Plasmid | Relevant Properties | Reference |
| Strains* | ||
| AM1-W | crtI::ISphoA/hah (i.e. crtI502) strain; white | [14] |
| C2925H | CmR, SmR; | New England Biolabs |
| CM253.1 | ΔmptG | [18] |
| CM501 | Isolate of wild-type M. extorquens AM1; pink | This study |
| CM502 | Isolate of AM1-W; white "wild-type" with crtI502 allele | This study |
| CM508 | Isolate of ΔmptG strain CM253.1 | This study |
| CM1122 | KmR; ΔhprA::kan; pCM431 integrated into CM501 | This study |
| CM1123 | KmR; ΔhprA::kan crtI502; pCM431 integrated into CM502 | This study |
| CM1203 | ΔhprA; kan deleted from CM1122 | This study |
| CM1204 | ΔhprA crtI502; kan deleted from CM1123 | This study |
| CM1263 | TcR, sucroseS; pCM441 integrated into CM501; crtI502 upstream; white | This study |
| CM1264 | TcR, sucroseS; pCM441 integrated into CM501; crtI upstream; pink | This study |
| CM1265 | TcR, sucroseS; pCM440 integrated into CM502; crtI upstream; pink | This study |
| CM1266 | TcR, sucroseS; pCM440 integrated into CM502; crtI502 upstream; white | This study |
| M. extorquens AM1 | RifR isolate; pink | [9] |
| Plasmids | ||
| pCM157 | TcR; broad-host-range cre expression vector | [4] |
| pCM184 | ApR, KmR, TcR; broad-host range cre-lox allelic exchange vector | [4] |
| pCM411 | ApR, KmR; mptG region cloned into pCR2.1 | This study |
| pCM417 | ApR, KmR; crtI region cloned into pCR2.1 | This study |
| pCM424 | ApR, KmR; ΔmptG region cloned into pCR2.1 | This study |
| pCM426 | ApR, KmR; crtI502 region cloned into pCR2.1 | This study |
| pCM428 | ApR, KmR; pCR2.1 with hprA upstream flank | This study |
| pCM429 | ApR, KmR; pCR2.1 with hprA downstream flank | This study |
| pCM430 | ApR, KmR, TcR; pCM184 with hprA upstream flank from pCM428 | This study |
| pCM431 | ApR, KmR, TcR; pCM430 with hprA downstream flank from pCM429 | This study |
| pCM432 | ApR, TcR; synthetic linker introduced into pCM184 | This study |
| pCM433 | ApR, CmR, TcR; pCM432 with sacB-cat fragment from pDS132; broad-host-range sacB-based allelic exchange vector | This study |
| pCM433r | ApR, CmR, TcR; pCM433 with sacB-cat fragment in the opposite orientation as in pCM433 | This study |
| pCM434 | ApR, KmR; hprA region cloned into pCR2.1 | This study |
| pCM435 | ApR, CmR, TcR; hprA region from pCM434 cloned into pCM433 | This study |
| pCM436 | ApR, CmR, TcR; mptG region from pCM411 cloned into pCM433 | This study |
| pCM437 | ApR, CmR, TcR; ΔmptG region from pCM424 cloned into pCM433 | This study |
| pCM438 | ApR, KmR; ΔhprA region cloned into pCR2.1 | This study |
| pCM439 | ApR, CmR, TcR; ΔhprA region from pCM438 cloned into pCM433 | This study |
| pCM440 | ApR, CmR, TcR; crtI region from pCM417 cloned into pCM433 | This study |
| pCM441 | ApR, CmR, TcR; crtI502 region from pCM426 cloned into pCM433 | This study |
| pCR2.1 | ApR, KmR; PCR cloning vector | Invitrogen |
| pDS132 | ApR, CmR;, sacB-based allelic exchange vector with λpir-dependent R6Kori | [10] |
| pRK2073 | SmR; helper plasmid supplying IncP tra functions | [17] |
*All M. extorquens AM1 strains are also RifR [9]. Antibiotic resistances are indicated as follows, Ap (ampicillin), Cm (chloramphenicol), Km (kanamycin), Rif (rifamycin), Sm (streptomycin), Tc (tetracycline).