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. Author manuscript; available in PMC: 2008 Aug 20.
Published in final edited form as: Biochemistry. 2006 Oct 24;45(42):12756–12766. doi: 10.1021/bi061169d

Figure 1.

Figure 1

The phosphopantetheinyl elimination fragments observed during tandem mass spectrometry. A) Pyrrolyl-S-PltL subjected to the tandem mass spectrometric method IRMPD. B) Enlargement of the mass spectral region that correspond to the smaller 1+ pantetheinyl fragment ion. C) Enlargement of the mass spectral region that correspond to the smaller 1+ phosphopantetheinyl fragment ion. D) Elimination fragment of mono-chloropyrrolyl-S-PltL E) Elimination fragment of dichloropyrrolyl-S-PltL. The protein derived ions are reported as the neutral monoisotopic mass while the PPant eliminated ions are reported as the 1+ monoisotopic mass. The remaining signals in the 450−500 m/z region are frequency spikes or amide backbone cleavage of the protein itself.