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Phosphopantetheinyl ejection as observed with an ion trap instrument. A) ESI/MS of holo-AcpK (10.3 kDa). B) Trap isolation of the 12+ charge state C) CAD at 17 % of the ions isolated in B. D) Broadband spectrum of HMG-S-PksL-2ACP (28 kDa). E) Trap isolation of the 31+ charge state F) CAD at normalized collision energy of 17 % of the ions isolated in E. 405.2 m/z is HMG-Pant. 261.1 m/z is Pant.
