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Coimmunoprecipitation of dynamin I and N-WASP with syndapin I. Rat brain high-speed supernatant was immunoprecipitated with affinity-purified antibodies against syndapin I or with unrelated rabbit IgG. Precipitated material was separated on SDS-PAGE, transferred to nitrocellulose, and analyzed with antibodies directed against syndapin I and dynamin I (A) or directed against N-WASP (B).
