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Melting temperatures and thermodynamic parameters* for folding of RQ and hybridization with complementary (P7C) and homologous (P7H) PNAs.
| −ΔG298K (kcal/mol) | −ΔH (kcal/mol) | −TΔS298K (kcal/mol) | Tm (°C) | |
|---|---|---|---|---|
| RQ | 2.7 ± 0.5 | 39.5 ± 1.1 | 36.8 ± 1.0 | 47.2 ± 1.0 |
| RQ + P7C | 12.3 ± 0.4 | 43.0 ± 0.4 | 30.6 ± 2.0 | 58.7 ± 0.8 |
| RQ + 2P7H | 28.5 ± 0.4 | 95.7 ± 1.9 | 67.1 ± 2.0 | 70.1 ± 0.5 |
Determined by curve fitting (41).
