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Biotin-labeled maleimide incorporation into the endogenous p53 protein in HCT116 cells was determined as described in Methods. Briefly, HCT116 cell extracts were exposed to 5mM Biot-maleimide with or without NEM pretreatment. 5 mM NEM was also added to terminate the reactions. p53 immunoprecipitates were prepared from these extracts and western blotted using the Strep-HRP reagent.
