Table 1.

Bovine pericardium (BP) water-soluble fraction proteins identified by two-dimensional western blotting with rabbit serum collected before or after exposure to BP

Spot Number	Protein Name	Accession N^o	Exposure Time Antibody First Detected	Protein Localization	MW	pI	Peptides Matched	Literature Evidence of Antigenicity
1	beta hemoglobin	gi\|27819608	0 d	Cytoplasm	15944.3	7.01	45	[51]
2	UBFD1 protein	gi\|151554567	0 d	Unknown	33143.6	5.55	1	None
3	albumin	gi\|162648	0 d	Cytoplasm	69278.5	5.82	19	[52]
or 3	hemoglobin beta-A chain	gi\|122539	0 d	Cytoplasm	15964.3	6.36	3	None
4	albumin	gi\|162648	0 d	Cytoplasm	69278.5	5.82	19	[52]
or 4	hemoglobin beta-A chain	gi\|122539	0 d	Cytoplasm	15964.3	6.36	3	None
5	albumin	gi\|162648	0 d	Cytoplasm	69278.5	5.82	19	[52]
or 5	hemoglobin beta-A chain	gi\|122539	0 d	Cytoplasm	15964.3	6.36	3	None
6	hypothetical protein LOC540335	gi\|134085635	21 d	Unknown	20765.6	5.09	3	None
7	apolipoprotein A-1	gi\|245563	21 d	Cytoplasm or Membrane	28414.8	5.57	16	None
8	apolipoprotein A-1	gi\|245563	21 d	Cytoplasm or Membrane	28414.8	5.57	9	None
9	glutathione S-transferase M1	gi\|28461273	21 d	Nuclear or Cytoplasm	25634.8	6.91	10	None
10	peroxiredoxin 2	gi\|27807469	56 d	Cytoplasm	21946.0	5.37	2	None
11	alpha-1-acid glycoprotein	gi\|94966811	56 d	Secreted	23182.4	5.62	1	None
12	triosephosphate isomerase	gi\|61888856	56 d	Cytoplasm	26672.8	6.45	2	[47]
13	hemoglobin chain A	gi\|116812902	56 d	Cytoplasm	15174.9	8.07	35	[51]
14	proline synthase homolog	gi\|77735663	56 d	Cytoplasm	29943.3	6.72	8	None
15	peroxiredoxin 3	gi\|75948233	56 d	Mitochondrial	31256.2	7.10	11	None
16	Ig gamma-2 chain C region (clone 32.2)	gi\|89611	56 d	Membrane	36019.8	8.04	11	None
17	hemoglobin beta-A chain	gi\|122539	56 d	Cytoplasm	15964.3	6.36	5	None
18	Enolase 1	gi\|87196501	56 d	Cytoplasm or Membrane	47296.4	6.37	9	[53, 54]
19	Enolase 1	gi\|87196501	56 d	Cytoplasm or Membrane	47296.4	6.37	21	[53, 54]
20	Enolase 1	gi\|87196501	56 d	Cytoplasm or Membrane	47296.4	6.37	37	[53, 54]
21	allergenbos d 6	gi\|1351907	56 d	Secreted	69248.4	5.82	43	[52]
or 21	hypothetical LOC534509	gi\|77736171	56 d	Unknown	52209.2	7.9	6	None
22	alpha-1-acid glycoprotein	gi\|94966811	56 d	Secreted	23182.4	5.62	1	None
23	haptoglobin	gi\|83638561	56 d	Cytoplasm	44830.6	7.83	7	None
24	parkinson disease 7	gi\|62751849	56 d	Unknown	20035.3	6.84	5	None
25	allergen bos d 6	gi\|1351907	56 d	Secreted	69248.4	5.82	18	[52]
26	allergen bos d 6	gi\|1351907	56 d	Secreted	69248.4	5.82	18	[52]
27	hypothetical LOC532481	gi\|129277510	56 d	Unknown	26176.5	6.59	10	None
28	hypothetical LOC532481	gi\|129277510	56 d	Unknown	26176.5	6.59	5	None
29	hypothetical LOC532481	gi\|129277510	56 d	Unknown	26176.5	6.59	17	None
30	hypothetical LOC532481	gi\|129277510	56 d	Unknown	26176.5	6.59	15	None
31	hypothetical LOC532481	gi\|129277510	56 d	Unknown	26176.5	6.59	3	None
32	beta hemoglobin	gi\|27819608	56 d	Cytoplasm	15944.3	7.01	7	[51]
33	haptoglobin	gi\|83638561	56 d	Cytoplasm	44830.6	7.83	7	None