Table 2.

Bovine pericardium (BP) lipid-soluble fraction proteins identified by two-dimensional western blotting with rabbit serum collected after exposure to BP

Spot Number	Protein Name	Accession N^o	Exposure Time Antibody First Detected	Protein Localization	MW	pI	Peptides Matched	Literature Evidence of Antigenicity
1	beta hemoglobin	gi\|27819608	21 d	Cytoplasm	15944.3	7.01	22	[51]
2	beta hemoglobin	gi\|27819608	21 d	Cytoplasm	15944.3	7.01	11	[51]
3	apolipoprotein A-I	gi\|245563	21 d	Cytoplasm or Membrane	28414.8	5.57	21	None
4	alpha-1-B glycoprotein	gi\|114053019	21 d	Secreted	53553.5	5.3	5	None
5	alpha-1-B glycoprotein	gi\|114053019	21 d	Secreted	53553.5	5.3	5	None
6	hemoglobin chain A	gi\|116812902	72 d	Cytoplasm	15174.9	8.07	16	[51]
7	hemoglobin chain A	gi\|116812902	72 d	Cytoplasm	15174.9	8.07	8	[51]
8	glutathione S-transferase pi	gi\|29135329	72 d	Nuclear or Cytoplasm	23613.1	6.89	2	None
9	hypothetical LOC614712	gi\|77736475	72 d	Unknown	18011.9	9.3	1	None
10	hypothetical LOC614712	gi\|77736475	72 d	Unknown	18011.9	9.3	1	None
11	hypothetical LOC614712	gi\|77736475	72 d	Unknown	18011.9	9.3	3	None
12	hypothetical LOC514335	gi\|77735877	72 d	Unknown	28699.1	8.76	4	None
13	hypothetical LOC532481	gi\|129277510	72 d	Unknown	26176.5	6.59	3	None
14	apolipoprotein A-I precursor	gi\|162678	72 d	Cytoplasm or Membrane	30271.3	5.8	3	None
15	apolipoprotein E	gi\|312893	72 d	Secreted	35869.7	5.44	3	None
16	annexin V	gi\|260137	72 d	Nucleus or Membrane	35942.7	4.94	6	[55, 56]
17	osteoglycin protein	gi\|74354996	72 d	Matrix	34197.4	5.43	5	None
18	osteoglycin precursor	gi\|129077	72 d	Matrix	34209.4	5.43	7	None
19	osteoglycin precursor	gi\|129077	72 d	Matrix	34209.4	5.43	8	None
20	osteoglycin precursor	gi\|129077	72 d	Matrix	34209.4	5.43	9	None
21	chain A dimethylarginine dimethylamino-hydrolase I	gi\|109157267	72 d	Cytoplasm	31157.6	5.67	5	[57]
22	chain D bovine mitochondrial F-1 ATPase	gi\|1827812	72 d	Membrane	51705.1	5.00	12	None
23	chain D bovine mitochondrial F-1 ATPase	gi\|1827812	72 d	Membrane	51705.1	5.00	20	None
24	hypothetical LOC535804	gi\|115497132	72 d	Unknown	56453.1	8.57	19	None
25	hypothetical LOC535804	gi\|115497132	72 d	Unknown	56453.1	8.57	19	None
26	allergen bos d 6	gi\|1351907	72 d	Secreted	69248.4	5.82	8	[52]