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. Author manuscript; available in PMC: 2008 Aug 20.
Published in final edited form as: Biochemistry. 2007 May 3;46(21):6299–6307. doi: 10.1021/bi700079w

Figure 3.

Figure 3

Gel electrophoresis analysis of apoA-I DMPC complexes. ApoA-I western blot of non-denaturing gradient gel electrophoresis was performed on complexes formed by incubation of DMPC LUVs with plasma-derived apoA-I. Lane 1, lipid-free apoA-I; lanes 2–4, overnight incubation mix of DMPC LUVs with apoA-I at a lipid:protein mole ratio of 200:1 (lane 2); 100:1 (lane 3); and 50:1 (lane 4); lane 5, peak II from gel filtration of DMPC:apoA-I mixture at 200:1 mole ratio; lane 6, peak III of gel filtration from DMPC:apoA-I mixture at 200:1 mole ratio; lane 7, peak IV from gel filtration of DMPC:apoA-I mixture at 50:1 mole ratio. 250 ng of protein was loaded each for lanes 1–4, and 5 µl of each isolated fraction was loaded without adjusting for protein concentration in lanes 5–7. The migration of a high molecular weight standard (Amersham Biosciences; Newark, NJ) containing proteins of known Stokes diameter (nm) is shown at the left.