Figure 5.

Kinetics of μ production. (A) The indicated cell lines (2 × 10⁷ cells) were incubated in [³⁵S]methionine-containing medium for various intervals. μ chains were analyzed by SDS-PAGE and then visualized by PhosphorImager. As described in Figure 4, 10⁷ cell equivalents were analyzed in the case of mutant cell lines, and 10⁶ cell equivalents were analyzed for the wild type. (B) PhosphorImager results presented in Table 2. Upper panel, results for both wild type (filled symbols) and Ter3 (open symbols); lower panel, Ter3 results on an expanded scale to show the nearly linear incorporation. Two independent experiments are plotted: the circles and triangles correspond to experiments 1 and 2 (Table 2), respectively.