Table 1.

Effect of mutations on the level of μs and μm RNA

Cell line	μs/actin					μm/actin
	Exp				Mean ± SD	Exp			Mean ± SD
	1	2	3	4		2	3	4
Original
WT	100	100	100	100	100	100	100	100	100
Ter3	55	22	80	75	58  ± 23	39	26	28	31 ± 6
Ter462*	103	90	138	100	108  ± 18	<0.1			<0.1
Recombinant
Ter3		18			18	37			37
Ter3/Ter462*			71	83	77  ± 6		<0.1	<0.1	<0.1

For each independent experiment (exp) total RNA was isolated from the indicated cell lines and analyzed by Northern blots using the probes defined in Figure 1 to detect μs, μm, and actin RNA, as shown in Figure 3. The radioactivity in each band was quantified by PhosphorImager, and the values obtained for μs and μm were divided by the value obtained for actin. The quotient obtained for each cell line is expressed as a percentage of the WT quotient. Experiments 2 and 3 correspond to the blots shown in Figure 3, A and B, respectively.