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. 2008 Jun 11;295(2):C414–C422. doi: 10.1152/ajpcell.00038.2008

Fig. 5.

Fig. 5.

Muscarinic inhibition of β-adrenergic response. A: time course of changes in total cAMP concentration in a ventricular myocyte exposed to a maximally stimulating concentration (0.2 μM) of Iso and subsequent addition of 10 μM acetylcholine (ACh). The concentration of cAMP detected by Epac2-camps was estimated using Eq. 1. B: averaged response to 0.2 μM Iso and Iso plus 10 μM ACh (P < 0.05). C: effects of Iso and ACh on the total cAMP concentration predicted by computational model of cAMP signaling in an adult ventricular myocyte (see methods). Note: baseline cAMP concentration, cAMP concentration in the presence of a maximally stimulating concentration of Iso, and cAMP concentration in the presence of Iso plus ACh are consistent with experimental observations.