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Hepatocyte production of MIP-2 is stimulated by HSP-72 and not contaminants. Primary C57BL/6 hepatocytes were treated with media, 11 pg/ml LPS, 1,000 ng/ml heat-denatured (boiled) HSP-72, or 1,000 ng/ml HSP-72 for 8 h. Supernatant was harvested and MIP-2 production was analyzed by ELISA. Data represent means ± SE of 4 experiments. *P < 0.05 compared with all other groups.
