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. 2008 Jun 11;295(2):C388–C393. doi: 10.1152/ajpcell.00178.2008

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Copyright © 2008, American Physiological Society

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Fig. 3. — FRET measurements from intact transgenic zebrafish embryos. A: epifluorescent images of control (a) and tnnt2 morpholino-injected (b) transgenic zebrafish embryos expressing YFP-CBD1-CFP at 2 days postfertilization. Arrow indicates the presence of pericardial edema in morpholino-injected fish. B: representative recording of changes in YFP (black) and CFP (gray) emission and the YFP-to-CFP ratio in an intact tnnt2 morpholino-injected YFP-CBD1-CFP transgenic zebrafish. Data were collected at 6 frames/s with a ×20 oil immersion lens.