Fig. 2.

Dependence of force on MLCK activation and RLC phosphorylation in bladder smooth muscle stimulated by KCl or CCh. A: MLCK activation and force were measured in bladder muscle strips from transgenic mice stimulated with 30, 40, 50, and 65 mM KCl (•, n = 5) or with 0.3, 1, 3, 10, and 30 μM CCh (○, n = 8). Measurements were obtained at 1 min. Contraction was normalized by the 65 mM KCl-induced contraction for each strip. B: force was measured in bladder muscle strips stimulated with concentrations of KCl (•, n = 5) or CCh (○, n = 5) as described in A. Strips were frozen at 1 min and processed for measurement of RLC phosphorylation. Values are means ± SE.