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Effects of Y27632 and calphostin C on phosphorylation of CPI-17 and MYPT1 in bladder strips in response to CCh. Strips were preincubated for 15 min with 10 μM Y27632 (Y+CCh) or 1 μM calphostin C (C+CCh) followed by 1 min with 10 μM CCh as indicated. Phosphorylations of CPI-17 (A) and MYPT1 (Thr694) (B) were assessed as described in Fig. 3. Values are means ± SE; n = 7–9. *P < 0.05 when compared with value at rest; #P < 0.05 when compared with CCh alone.
