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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Insect Biochem Mol Biol. 2008 Apr 22;38(7):705–713. doi: 10.1016/j.ibmb.2008.04.002

Figure 5.

Figure 5

Qualitative analysis of 3xP3-sensor mosquitoes following knock-down of putative RNAi genes. (A) 3xP3-sensor mosquitoes (line #2) were scored based on intensity of EGFP fluorescence. The percent of total mosquitoes scored at each value (1–4) is plotted, with the average for each sample and the total number of mosquitoes scored (n) given in parentheses. Results represent the sum of three replicate experiments performed during the G4-G8 generations for uninjected, dsSINV-DCR-2 and dsSINV-AGO-3 and two replicates for dsSINV-AGO-2. Sample photographs below the graph illustrate the approximate scoring guide. (B) Northern analysis of 3xP3-sensor mosquitoes following knock-down of putative RNAi genes. RNA from uninjected, dsSINV-AaDCR-2, or dsSINV-AaAGO-3 injected heads was probed for EGFP mRNA. Gel photograph of rRNA prior to transfer serves as loading control. (C) Real-time RT-PCR (two-step) to detect EGFP mRNA expression in 3xP3-sensor #2 heads following knockdown of AaDCR-2 or AaAGO-3. Samples were compared to EGFP expression in uninjected 3xP3-sensor #2 mosquito heads (−).