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PT S1 Degradation by the 20S Proteasome. (A) Reduced PT S1 and the reduced CT A1/CT A2 heterodimer were incubated at 37°C with the 20S proteasome. Toxin samples taken at the indicated time points were visualized by SDS-PAGE and Coomassie staining. (B) PT S1, PT, and PT B were incubated at 37°C with the 20S proteasome under reducing conditions. Toxin samples taken after 0 or 20 hrs of incubation were visualized by SDS-PAGE and Coomassie staining.
