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. 2008 Sep;57(9):2413–2420. doi: 10.2337/db07-1323

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Copyright © 2008, American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 7. — Effects of HES1 shRNAs TRCN18990 and TRCN18991 on gene expression in cultured islet cells. A: Immunoblotting for HES-1 in protein extracted from islet cells 14 days following infection with HES1 shRNA TRCN18990, TRCN18991, or nontarget virus. HSC70 served as a loading control. B: qPCR analysis of RNA extracted from islet cells 14 days following infection with HES1 shRNAs or nontarget virus. RQ indicates relative quantification compared with cells infected with nontarget virus. Data are means ± SD (n = 3). Significant differences compared with nontarget are indicated by asterisks. ▪, nontarget; □, TRCN18990; , TRCN18991.

Inline graphic — Effects of HES1 shRNAs TRCN18990 and TRCN18991 on gene expression in cultured islet cells. A: Immunoblotting for HES-1 in protein extracted from islet cells 14 days following infection with HES1 shRNA TRCN18990, TRCN18991, or nontarget virus. HSC70 served as a loading control. B: qPCR analysis of RNA extracted from islet cells 14 days following infection with HES1 shRNAs or nontarget virus. RQ indicates relative quantification compared with cells infected with nontarget virus. Data are means ± SD (n = 3). Significant differences compared with nontarget are indicated by asterisks. ▪, nontarget; □, TRCN18990; , TRCN18991.