Table 1.
EGF receptor mutants used in Ab P2 binding studies
| Cell type | Receptor type | Number of receptors per cell (×105) |
|---|---|---|
| NIH3T3 | Untransfected | 0.025 |
| CL17 | Wild-type | 1.41 |
| MI31 | Y1173F | 0.56 |
| MI32 | Y1148F | 2.49 |
| MI33 | Y(1173-1148)F | 0.70 |
| MI34 | Y(1173-1148-1068)F | 0.77 |
| MI35 | Y(1148-1068)F | 0.25 |
| MI36 | Y(1173-1068)F | 0.45 |
| MI37 | Y1068F | 1.85 |
| MI38 | Y1086F | 0.25 |
| MI40 | Y992F | 0.61 |
| MI41 | Y(1173-1148-1086-1068-992)F | 0.50 |
| Dc63 | C-terminal deletion of 63 amino acids lacking tyrosine 1173 and 1148 | 0.39 |
The receptor sites per cell were determined by 125I-EGF binding according to the method described in MATERIALS AND METHODS. The amino acids are identified by their single letter codes: F for Phe, Y for Tyr.