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. 2008 Jul 23;3(7):e2749. doi: 10.1371/journal.pone.0002749

Figure 8. Isolated eyes show rhythmic expression of xBmal1 at stage 40 but not at stage 31.

Figure 8

Eyes were dissected from embryos maintained in a 12L:12D cycle at different stages of development and different circadian times (ZT 0 (dawn), ZT6 (mid-day), ZT12 (dusk),and ZT18 (midnight)). The eyes were analyzed by qRT-PCR. The relative quantitation (RQ) of xBmal1 for each sample was calculated with respect to EF1α. No difference in the levels of xBmal1 expression was observed in stage 31 embryonic eyes at any time of day tested (ANOVA; df3, F = 1.77, p = 0.176; arrhythmic). A significant difference in xBmal1 expression was observed when all ZTs were analyzed in stage 40 embryonic eyes (ANOVA; df3, F12.23, p = 0.00009). The asterisk shows that the level of xBmal1 expression at ZT18 was significantly different from ZT0 (ANOVA, df1, F = 27.82, p = 0.0004). Bars in each graph denote standard error.