Figure 1.

γ-Synuclein and Brn-3a localization were examined in the human retina by immunofluorescence. γ-Synuclein (red) was present in the cytoplasm of retinal ganglion cells (RGCs; triangles) and in the nerve fiber layer (NFL), while Brn-3a (green, arrowheads) was observed in RGC nuclei. Arrows mark cells in the retinal ganglion cell layer (GCL) that were not stained by either Brn-3a or γ-synuclein. Nuclei throughout the retina are counterstained with DAPI (blue), and little immunofluorescence is noted through the inner or outer plexiform layers (IPL and OPL, respectively), or inner and outer nuclear layers (INL and ONL, respectively). Thus γ-synuclein is localized both in the body of RGC and in their axons.