Figure 2.

Increased LAMP-1 content in MPR-negative cells. Aliquots of 10 μg protein of normal or MPR-negative fibroblast total cell lysates were boiled in 1× SDS-sample buffer, resolved on polyacrylamide gels, and transferred to nitrocellulose. The blot was probed with the anti-mouse LAMP-1 mAb 1D4B and the anti–AP-1 ς1 subunit antibody, DE/1. The nomenclature used to indicate the presence of the MPRs is +/+ for the normal cells, and −/− for the CD- and CI-MPR double-negative cells. Only the relevant portions of the blot are shown. The faint band seen above the ς1 subunit might represent ς1B (Takatsu et al., 1998).