Table 1.
Pentalenolactone (ptl) gene cluster of Streptomyces avermitilisa
| Gene | ID | Start nt | End nt | Definition | Best matchb |
|---|---|---|---|---|---|
| gap1 | SAV2990 | 3,745,497 | 3,746,501 | glyceraldehyde-3-phosphate dehydrogenase | ptlK, pentalenolactone resistance gene |
| ptlH | SAV2991 | 3,746,862 | 3,747,719 | putative hydroxylase | PF05721: Phytanoyl-CoA dioxygenase (PhyH) |
| ptlG | SAV2992 | 3,747,777 | 3,749,231 | putative transmembrane efflux protein | PF07690: Major Facilitator Superfamily |
| ptlF | SAV2993 | 3,749,307 | 3,750,119 | putative oxidoreductase | PF00106: short chain dehydrogenase |
| ptlE | SAV2994 | 3,750,177 | 3,751,961 | putative monooxygenase | PF00743: Flavin-binding monooxygenase-like |
| ptlD | SAV2995 | 3,751,962 | 3,752,882 | putative dioxygenase | PF02668: Taurine catabolism dioxygenase TauD, TfdA family |
| ptlC | SAV2996 | 3,752,934 | 3,753,110 | hypothetical protein | |
| ptlB | SAV2997 | 3,753,107 | 3,754,120 | polyprenyl diphosphate synthase | probable farnesyl diphosphate synthase (idsA2) |
| ptlA | SAV2998 | 3,754,725 | 3,755,735 | pentalenene synthase | PF03936: Terpene synthase family, metal binding domain |
| ptlI | SAV2999 | 3,755,792 | 3,757,141 | cytochrome P450 | CYP183A1 |
| ptlR | SAV3000 | 3,757,201 | 3,757,662 | putative AraC-family transcriptional regulator | PF00165: Bacterial regulatory helix-turn-helix proteins, AraC family |
| ptlJ | SAV3001 | 3,757,659 | 3,758,093 | putative lyase | PF00903: Glyoxalase/Bleomycin resistance protein, Dioxygenase superfamily |
| ptlL | SAV3002 | 3,758,163 | 3,758,933 | hypothetical protein | Nfa37730 of Nocardia farcinica IFM 10152 |
a
For detailed annotation and alignments, see the Streptomyces avermitilis Genome Project website: http://avermitilis.ls.kitasato-u.ac.jp/
b
PF Number is the NCBI PFAM reference.