Fig. 5.

Detection of changes in NAADP concentration under cell extract conditions. The displacement curve was generated from known NAADP concentrations (blue squares). The difference between known NAADP concentrations spiked into rat brain extract (prepared as described in text) is correctly detected (red circles). Error bars represent standard errors of the mean (n = 3). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)