Fig. 6.

Improvement of NAADP detection in cell extract samples. The green trace shows standards made in intracellular medium (Glu-IM), the blue trace shows standards in “acid-extracted” intracellular medium, and the red trace shows standards in intracellular medium supplemented with 1 M potassium acetate. Error bars represent standard errors of the mean (n = 3). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)