Table 1.

The sTF_2-219 mediated improvement in the amidolytic activity of fVIIa

STF2-219	Maximal velocity mOD/min	Kd(app) nM
WT	9.2 ± 0.4	2.9 ± 0.7
N199A	8.0 ± 0.2	3.0 ± 0.5
R200A	7.7 ± 0.2	3.2 ± 0.6
K201A	7.3 ± 0.2	2.9 ± 0.7

The amidolytic function of 5 nM fVIIa toward SpVIIa in the presence of increasing concentrations of wild-type or mutant cofactors was determined in TBS/Ca²⁺ as described under “Materials and Methods”. All values are average of three measurements. The amidolytic activity of fVIIa in the absence of sTP_2-219 was not significantly different from the background chromogenic substrate activity in the absence of the protease.