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. 2008 Aug 25;182(4):685–701. doi: 10.1083/jcb.200803137

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© 2008 Axe et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Autophagosome maturation after omegasome exit. Cells expressing GFP-DFCP1 and mRFP-MAP-LC3 were starved and imaged for the indicated time interval. At 30 min after starvation, the cells were also incubated with 2 μM MDC. Note that an autophagosome emerges first from an omegasome (panels labeled autophagosome formation; arrow indicates the omegasome) and then begins to stain with MDC (panels labeled autophagosome maturation) without appearing to change its appearance or to fuse with another MDC-positive vesicle.