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. 2008 Jun 26;198(3):499–509. doi: 10.1677/JOE-08-0019

Figure 2.

Figure 2

SKA2 is a 14 kDa protein expressed in SCLC and non-SCLC cell lines. (a) Western blot showing SKA2 protein expression using novel anti-peptide antibody. The SKA2 immunogenic peptide (lane 1), endogenous SKA2 in A549 cell lysate (lane 2), and recombinant bacterially expressed GST-SKA2 (lane 3) were detected as bands of 8, 14 and 42 kDa, as predicted. Peptide-blocked antibody controls are also shown, as indicated. (b) Western blot showing SKA2 expression in cell lines and tissues. A549 human non-SCLC cells, normal human lung tissue, DMS79 human SCLC cells, CORL24 human SCLC cells, HepG2 human liver carcinoma cells, mouse liver tissue, 3T3L1 mouse fibroblast cells, HEK293 human embryonic kidney cells, CEM-7A human T-lymphoblast cells, Rat-1 rat fibroblast cells, primary human skin fibroblast cells, AtT20 mouse pituitary cells, COS7 transformed monkey kidney fibroblast cells and GH3 rat pituitary cells. (c) Western blot showing SKA2 expression in human breast cancer cell lines MCF-7, MCF-10A and MDA-MB231, by comparison with A549 and HeLa cells. (d) SKA2 protein expression in A549 and HEK293 cells transfected with empty expression vector (control) and with cmyc-SKA2. Endogenous SKA2 is a 14 kDa protein and the exogenous cMyc-SKA2 is an 18 kDa protein.