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. 1999 Mar;10(3):567–580. doi: 10.1091/mbc.10.3.567

Figure 10.

Figure 10

(A) Effect of anti-Ku70 and anti-Ku86 antibodies on the in vitro replication of p186. Increasing amounts (3.5, 7.0, and 14 μg) of anti-Ku70 (black bars), anti-Ku86 (white bars), and goat IgG (dashed bars) antibodies were preincubated with HeLa cell extracts, before the addition of the plasmid DNA template (p186, 200 ng). (B) Neutralization of the anti-Ku70 and anti-Ku86 antibodies. A constant amount (14 μg) of the anti-Ku70 (black bars) and anti-Ku86 (white bars) antibodies was neutralized using specific (lane 3) or nonspecific (lane 4) peptides before being added to the reaction. Lane 1 represents the p186 control reaction, and lane 2 represents the reaction inhibited with 14 μg of antibodies. In vitro reactions were performed, and the data were processed as described in MATERIALS AND METHODS.