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Specificity of OBA binding to the A3/4 sequence. Band-shift reactions were performed by incubating constant amounts of radioactively labeled A3/4 DNA (0.5 ng) and pool F (200 ng). Increasing molar excess (50, 500, 1000, and 2000×) amounts of the A3/4 sequence, either ds or leading and complementary A3/4 single strands, were used as cold competitors.
