Figure 8.

Western blot analysis of the band-shifted complexes generated with apOBA. (I) Band-shift reactions were performed under regular conditions, using 0.25 ng/reaction of A3/4 radioactive probe and increasing amounts of apOBA (100, 250, and 350 ng, respectively). A control reaction was performed in which the DNA was absent. A duplicate set of reactions was also performed using nonradioactive A3/4 as probe. All reactions were subjected to electrophoresis in a minigel apparatus so that the DNA probe ran out of the gel. The nonradioactive half of the gel was transferred to a membrane and subjected to Western Blotting analysis with (II) anti-Ku86 antibody and (III) anti-Ku70 antibody. The position of the main OBA–A3/4 (asterisk) and of the faster migrating (arrow) complexes is indicated.