Figure 2.

Condensin mutants release tgm silencing. Strains containing mutations in each condensin subunit were transformed with a plasmid containing a tgm silencing test construct (shown in A) (Hull et al. 1994; Kendall et al. 2000; L. Wang et al. 2005), where the SUP4 tRNA gene silences HIS3 expression in wild-type cells. Cells with defective tgm silencing are able to grow on media lacking histidine (SGR-Ura-His) (B). Growth at semi-permissive temperature for the temperature-sensitive mutants (30°C) is shown, although growth at 23°C gave similar results (data not shown). All five condensin mutants release tgm silencing to varying extents, similar to deletions of RNA polymerase I components Δrpa12 and Δrrn10, strains that are known to release tgm silencing (L. Wang et al. 2005).