Figure 6.

Interphase adhesion pattern controls the bipolar/multipolar decision. (A) Bright-field images representing the different interphase cell shapes of MDA-231 cells: elongated, polarized, and round. (B) Elongated cell morphology is correlated with bipolar division. Quantitation of bipolar and multipolar divisions by live-cell imaging of MDA-231 cells with different interphase shapes (n = 279; 45% elongated, 46% polarized, 9% round). (C) RF position correlates with bipolar or multipolar divisions. Representative images from DIC movies of 4N BSC-1 cells from interphase (left) through mitosis (right). Yellow asterisks indicate RFs. Sixty-six binucleated cells were imaged in three independent experiments. (D) Interphase cell shape controls spindle morphology in cells with extra centrosomes. MDA-231 cells were plated on FN micropatterns (H, O, and Y) and stained for MTs (α-tubulin, green), centrosomes (pericentrin, red), and DNA (blue). (E) Percentage of cells with multipolar spindles in MDA-231 cells after plating on different patterns (H, O, and Y). Note that the percentage of multipolar spindles was quantified from all metaphase cells. Controls were obtained from cells that were attached to unconstrained FN micropatterns. Graph represents three independent experiments. Bar, 10 μm.