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. 2008 Aug 15;22(16):2172–2177. doi: 10.1101/gad.1699608

Figure 4.

Figure 4.

Cooperativity of Rheb overexpresison and Pten haploinsufficiency. (A) Incidence of HGPIN in the AP from five 10-mo-old mice of each genotype. (B) Histopathological (H&E) analysis of the AP sections from 10-mo-old mice of each genotype. (C) IHC staining of prostate sections from 5-mo-old mice of each genotype with an anti-phospho S6 antibody. (D) Western blot analysis of protein extracts from AP of 5-mo-old mice of the indicated genotype. (E) Western blot analysis of protein extracts from mice of the indicated genotype treated or not with RAD001. (F) Ki-67 staining on prostate sections from 6-mo-old mice of the indicated genotypes. Bottom panel: quantification of Ki-67. A representative count in triplicate out of three with similar results is shown with standard deviations. (G) Molecular model of mTORC1 activation by Rheb leading to senescence and proliferation and feedback inhibition of the PI3K pathway.