Table 1.
Analysis of ALMC-1 and ALMC-2 IgG and free λLC secretion rates
| Assay, experiment no. | ALMC-1, pg Ig secreted/cell per 24 h |
ALMC-2, pg Ig secreted/cell per 24 h |
||
|---|---|---|---|---|
| Nil | IL-6 | Nil | IL-6 | |
| IgG | ||||
| 1 | 5.0 | 8.0 | 20.9 | 43.3 |
| 2 | 6.2 | 9.1 | 15.2 | 36.5 |
| Free λLC | ||||
| 1 | 0.24 | 0.16 | 0.6 | 0.8 |
| 2 | 0.71 | 0.47 | 0.81 | 0.9 |
ALMC-1 and ALMC-2 cells were cultured at 106 cells/ml with or without (Nil) IL-6 for 24 hours. Cell-free supernatants were then collected, and total IgG and free λLC levels were determined using ELISA and expressed on a per-cell basis.