Table 4.

Cytogenetic analysis of ALMC-1, ALMC-2, and primary patient cells

Cells	Chromosome no.	Representative karyotype	c-MYC amplification, % cells	p53 deletion, % cells
Primary patient before PBSCT	ND	ND	30	40
ALMC-1	74-80	X,-X,-X,-X, add(1)(p13)x2,-2, der(2)t(1;2)(p13;q33), der(3)t(1;3)(q21;q27)x2, add(3)(q12),-4, add(5)(p13)-8,-8,-10, add(11)(q13), add(11)(q21)x2,-13,-13,-14,-14,t(14;20)(q32;q11.2)x2,-16,-16, add(18)(p11.2)x2,-21,-22,-22,-22,+6marX,-X,a dd(1)(p13),der(2)t(1;2)(p32;q37), add(3)	100	100
Primary patient after PBSCT	38-43	(q27),der(6;22)(p10;q10), add(7)(p22),i(7)(p10), add(8)(p11.2),t(8;14)(q24.1;q11.2), add(10)(p13), add(11)(q23), add(12)(p13),-13, add(14)(q32), der(16) add(16)(p13.1) add(16)(q22),-19,-20,-20,-21,-22,+0–1r,+0–1mar	100	100
ALMC-2	39-40	X,-X, add(1)(p13), der(2)t(1;2)(p13;q33), add(3)(q13.2), add(3)(q27), +add(5)(p13),-6, der(6) add(6)(p21.3) add(6)(q13), add(7)(p15),-8,-8,-10, add(11)(q21),-13,-14,-14,-16, der(16) add(16)(p13.1) add(16)(q22), add(17)(p11.2),-19,-20,-22,+5mar	100	100

ALMC-2 and post-PBSCT primary cells display hypodiploid DNA content and the ALMC-1 cells are near-tetraploid. The karyotypes shown are representative of those observed in 10 to 15 abnormal metaphases. c-MYC amplification and p53 deletion were measured by interphase FISH.

ND indicates not done.