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. 2008 Aug 5;8:134. doi: 10.1186/1471-2180-8-134

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Copyright © 2008 Hobson et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A ΔhsdR mutant is unaffected in virulence factor production. E2348/69 and NH4 were grown under conditions that stimulate virulence factor production and BfpA (a) levels were measured by western analysis of whole cell lysates. Secreted Tir (b) levels were measured by western analysis of SDS-PAGE gels after electrophoresis of precipitated supernatant samples. (c) Levels of a cross-reactive protein serve as a loading control. The experiment was performed twice and one experiment is shown.