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. 2008 Aug 5;8:134. doi: 10.1186/1471-2180-8-134

Table 1.

Transformation efficiencies of E2348/69 vs. E2348/69 ΔhsdR

PLASMID DESCRIPTION SIZE ORI COPY# TRANSFORMATION EFFICIENCIESa






E2348/69b E2348/69c
pJW15 lux reporter ~10 kb p15A 10–12 2.5 × 10-11 8.3 × 10-5
pNLP10 lux reporter ~10 kb pSC101 ~5 0 5.4 × 10-5
pLAFR1 cosmid cloning vector 21.6 RK2 5–7 3.0 × 10-9 9.0 × 10-7

a. Transformation efficiencies are expressed as the (number of transformants/number of viable cells)/ug DNA. All experiments were repeated at least three times, and one representative experiment is shown.

b. Transformation efficiencies observed with plasmid isolated from E. coli K-12.

c. Transformation efficiencies observed with plasmid isolated from E2348/69ΔhsdR.