Table 1.
PLASMID | DESCRIPTION | SIZE | ORI | COPY# | TRANSFORMATION EFFICIENCIESa | |
E2348/69b | E2348/69c | |||||
pJW15 | lux reporter | ~10 kb | p15A | 10–12 | 2.5 × 10-11 | 8.3 × 10-5 |
pNLP10 | lux reporter | ~10 kb | pSC101 | ~5 | 0 | 5.4 × 10-5 |
pLAFR1 | cosmid cloning vector | 21.6 | RK2 | 5–7 | 3.0 × 10-9 | 9.0 × 10-7 |
a. Transformation efficiencies are expressed as the (number of transformants/number of viable cells)/ug DNA. All experiments were repeated at least three times, and one representative experiment is shown.
b. Transformation efficiencies observed with plasmid isolated from E. coli K-12.
c. Transformation efficiencies observed with plasmid isolated from E2348/69ΔhsdR.