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. 2008 Sep 3;3(9):e3115. doi: 10.1371/journal.pone.0003115

Figure 4. Clathrin knockdown in HEK293 cells leads to an increase in the G2/M phase content.

Figure 4

A) Western blot analysis of HEK293 cells transfected with siRNA against CHC17 or with scrambled siRNA. β-actin was used as a loading control and two different clathrin heavy chain antibodies raised against the N-terminal domain (TD.1; Abcam) and the C-terminus (Ab21679; Abcam) were used on cell lysates harvested at the time of cell cycle analysis. B) The cell cycle profiles of scrambled siRNA treated and CHC siRNA treated cells are shown. The percentage of cells in each cell cycle phase was calculated by ModFit Sofware and is shown in the boxed legend.