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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Cancer Res. 2008 Jun 1;68(11):4068–4076. doi: 10.1158/0008-5472.CAN-07-5667

Figure 3. Aberrant cell cycle progression is sustained in RB ablated lungs 9 months after a single episode of injury and is associated with increased apoptosis.

Figure 3

Quantitative data for cell cycle markers and TUNEL analysis on RB ablated and control adult lungs from mice treated with doxycycline throughout gestation. BrdU incorporation and PH3 and Ki67 expression were assessed by immunohistochemistry 9 months after injury. Quantified data is presented as average percent positive cells + SEM (A). RB ablated lungs showed a statistically significant increase in S-phase cells (BrdU), mitotic cells (PH3) and overall proliferation (Ki67) as compared to controls (*p=0.04, *p=0.01 and *p=0.02, respectively). Apoptosis was assessed by TUNEL assay on lung sections before injury (day 0) and on day 4, day 14 and 9 months after injury. Representative results from the 9 month time point are shown for RB ablated (B) and control (C) lungs. Percent TUNEL positive cells (arrow) were quantified and data represented as average percent positive cells + SEM (D). Apoptosis was increased in RB ablated lungs as compared to controls at all time points analyzed with the increase reaching statistical significance on day 14 (*p=2.9×10−5) and 9 months (*p=0.006) after injury. Data is representative of 5–11 animals per time point. br=bronchiole; Original magnification: 1000×