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. Author manuscript; available in PMC: 2008 Aug 22.
Published in final edited form as: Chem Res Toxicol. 2007 Mar 27;20(4):565–571. doi: 10.1021/tx700023z

Figure 2.

Figure 2

Relationship of added to detected Acr-dGuo. Various amounts of α-OH- and γ-OH-Acr-dGuo standards (17, 34, 85, 170, and 340 fmol) were added to calf thymus DNA (0.5 mg) and analyzed by the method described in the text. Background levels in calf thymus DNA (40 adducts/109 nucleotides for α-OH-Acr-dGuo, and 30 adducts/109 nucleotides for γ-OH-Acr-dGuo) were subtracted from each amount detected. Each point represents a triplicate measurement. A, α-OH-Acr-dGuo, R2 = 1.0; B, γ-OH-Acr-dGuo, R2 = 1.0.