Fig. 3.

Representative pictures of In situ nick-end labeling (TUNEL) staining techniqueTUNEL staining in spinal cord sections and the numbers of TUNEL positive cells. Values are expressed as mean ± SEM for each group. Significant differences among the groups were analyzed with the Kruskal–Wallis test (*P < 0.05), and significant differences between the GP group and the MP or MC group were analyzed with the Mann–Witney U test (†P < 0.05, vs the MP and MC group). (N = 8, MP methylprednisolone, MC minocycline, GP pregabalin)