Figure 3.

Electrophoretic mobility shift of XMAP4 in mitosis. Interphase (I) and mitotic (M) cell extracts of nontransfected A6 cells (−) and A6 cells expressing wild-type GFP–XMAP4 (WT) or GFP–CM10A (CM10A) at subconfluence were immunoblotted with anti-XMAP4 antibody. Arrowheads and arrows denote endogenous XMAP4 and wild-type GFP–XMAP4 and GFP–CM10A, respectively. Electrophoretic mobility shift in mitosis was observed within two arrows or two arrowheads. This antibody recognized upwardly shifted XMAP4 bands more intensely, although the reasons for this are not known.