Figure 5.
Impaired anaphase chromosome separation in A6 cells expressing GFP–CM10A. Living A6 cells expressing GFP–WT (A and B) or GFP–CM10A (C–F) were observed. Left, differential interference contrast images; middle, GFP fluorescence images; right, DNA staining images with DAPI (2.5 μg/ml). (A) normal metaphase; (B) normal anaphase A; (C) BFP movement; (D) spindle elongation without chromosome separation; (E) cytokinesis without chromosome separation; (F) incomplete cytokinesis with decondensed chromosomes. Arrowheads in E and F denote cleavage furrows. Bar, 10 μm.