Table 1.
Mutations of serine and threonine residues in potential phosphorylation sites to alanine
| Consensus
|
p34cdc2 kinase [(S/T)PX(K/R)]
|
MAP kinase [PX(S/T)P, PXX(S/T)P]
|
p34cdc2 kinase and MAP kinase
|
MARK [KXGS]
|
|---|---|---|---|---|
| Residues | S437,T752,S771, T795,T823,T877 | T630,T756 | S741,S827 | S1001,S1075, S1111,S1132 |
| Wild type | ||||
| WT | – | – | – | – |
| Mutant | ||||
| CM10A | A | A | A | – |
| C6A | A | – | – | – |
| CM8A | A | – | A | – |
| R4A | – | – | – | A |
Four XMAP4 mutants were produced by site-directed mutagenesis. A, serine and threonine residues were mutated to alanine; –, not mutated.